The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
คณะวิทยาศาสตร์
This project presents the development of a "Smart Cat House" using Internet of Things (IoT) and image processing technology to facilitate and enhance the safety of cat care for owners. The infrastructure of the smart cat house consists of an ESP8266 board connected to an ESP32 CAM camera for cat monitoring, and an Arduino board that controls various sensors such as a motion sensor in the litter box, a DHT22 temperature and humidity sensor, an ultrasonic water and food level sensor, including a water supply system for cats, an automatic feeding system, and a ventilation system controlled by a DC FAN that adjusts its operation according to the measured temperature to maintain a suitable environment. There is also an IR sensor to detect the cat's entry into the litter box and an automatic sand changing system with a SERVO MOTOR. All systems are connected and controlled through the Blynk application, which can be used on mobile phones, allowing owners to monitor and care for their pets remotely. Cat detection and identification uses image processing technology from the ESP32 CAM camera in conjunction with YOLO (You Only Look Once), a high-performance object detection algorithm, to detect and distinguish between cats and people. Data from various sensors are sent to the Arduino board to control the operation of various devices in the smart cat house, such as turning lights on and off, automatically changing sand, adjusting temperature and humidity, feeding food and water at scheduled times, or ventilation. The use of a connection system via ESP8266 and the Blynk application makes it easy and convenient to control various devices. Owners can monitor and control the operation of the entire system from anywhere with internet access.
คณะวิทยาศาสตร์
This research will begin with a review of literature and related studies to examine existing technologies and methods for hand gesture recognition and their applications in controlling electronic devices such as drones, robots, and gaming systems. Subsequently, a hand gesture recognition system will be designed and developed using machine learning and computer vision techniques, with a focus on creating an algorithm that operates quickly and accurately, making it suitable for real-time control. The developed system will be tested and refined using various simulated scenarios to evaluate its efficiency and accuracy in diverse environments. Additionally, a user-friendly interface will be developed to ensure accessibility for all user groups. The research will also incorporate qualitative studies to gather feedback from both novice users and experts, which will contribute to further system improvements, ensuring it effectively meets user needs. Ultimately, the findings of this research will lead to the development of a functional prototype for gesture-based control, which can be applied in industries and entertainment. This will contribute to advancements in innovation and new technologies in the future.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
In the world of blood donation, there are 2 types of people: those who donate blood and those who don't. Most campaigners emphasize how to persuade more people to donate blood and recruit more new blood donors. We believe that even though such focus is important, there're more critical aspects that might have been neglected, which is: for those who have already made up their minds to be blood doners, will they be successful in donating when the time comes? According to our studies, only 63 % of attempted doners are successful. Regrettably, 37 % has to go home disappointed as their bodies are not fit for the conditions required by Red Cross medical staff at blood donation centers (which include some most basic preparations such as low-fat food intake and 8-hours sleep on the night before). Our campaign, ‘Blood in Need, Buddy Indeed’, focuses on 2 aspects. Firstly, to persuade more people to donate blood. Secondly, for those who have made up their minds to donate blood, we will provide necessary support (both body and mind) so that they are fully prepared and successful in donating blood when the time comes via networks of systems, staffs and the newly designed and prototype of the application ‘Blood D’. Our campaign covers the whole ‘before/during/after’ experience of users (as blood doners). Support includes assessment of their current condition whether they are within the requirement of Red Cross Blood Bank. ‘Blood D’ will also provide relevant information on blood donating events, such as locations, and time booking. Once sign-up, the application “Blood D” will sent friendly reminder and clear infographic on how to prepare their bodies as daily notifications during the 7 days countdown. This is to ensure that the users’ blood will be ‘D’ (homophone of the Thai word ‘ดี’ which mean ‘good’ and at the same time playing on the word ‘ Buddy’) or be the ‘good blood’ that can save lives for those in need. After organizing 4 blood donation events both within and outside the KMITL. The numbers of successful blood doners have increased from 63 % to 78 % (this number is the average of 4 events, with the most successful event of 89%). The campaign has won the first runner up in national blood donation campaign competition. It is highly anticipated that once the application “Blood D” is fully launched, it will help increase the amount of blood collected up to 15% with the same numbers of existing doners.