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คณะวิทยาศาสตร์
This study aimed to develop an anti-acne gel formulation using partially purified Limnophila aromatica extract and to evaluate its antibacterial efficacy, stability, and safety in accordance with international standards. The raw material was extracted using 99.9% v/v ethanol and partially purified via ion-exchange resin column chromatography. The resulting extract was formulated into a Carbopol 940 base gel at varying concentrations. The results demonstrated that the extract exhibited potent inhibitory and bactericidal activities against Cutibacterium acnes (MIC/MBC = 14.26 µg/mL), performing twice as effectively as Centella asiatica extract and comparably to Garcinia mangostana peel extract. In formulation testing, the L. aromatica acne gel selectively inhibited C. acnes without disrupting the normal skin microbiota, Staphylococcus epidermidis. Accelerated stability testing (at 40 °C and 50 °C for 3 months) revealed excellent formulation stability, with no phase separation or precipitation and a stable pH of 5.9–6.1, indicating a shelf life of 1 year. Regarding safety, the product met international standards (FDA BAM (2024), ISO 22717:2015, ISO 22718: 2015, USP 46/NF41:2023 และ USP 44), showed no cytotoxicity toward human HaCaT keratinocytes (IC50 > 1000 µg/mL), and induced no skin irritation in a human volunteer panel of 30 subjects (Mean Irritation Index = 0.00). In conclusion, the L. aromatica acne gel demonstrates an optimal balance of efficacy, stability, and safety. Free from parabens, silicones, and synthetic fragrances, this formulation is fully prepared for technology transfer and commercialization in the cosmeceutical industry.
คณะอุตสาหกรรมอาหาร
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คณะวิศวกรรมศาสตร์
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